KMID : 0620920060380010100
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Experimental & Molecular Medicine 2006 Volume.38 No. 1 p.100 ~ p.106
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Naltrexone influences protein kinase Ce and integrin a7 activity in SH-SY5Y neuroblastoma cells
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Oh DY
Jung KH/Yang BH/Lee JS/Choi IG/Chai YG
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Abstract
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Alcohol influences the neuroadaptation of brain cells where receptors and enzymes like protein kinase C (PKC) exist. Naltrexone acts on opioid receptors. However, other mechanisms of action remain unknown. We prepared SH-SY5Y neuroblastoma cells, and fed them with 150 mM ethanol for 72 h followed by treatment with naltrexone for 24 h. We performed microarray analysis and reverse trans - criptase-polymerase chain reaction. Our results showed that PKC¥å increased 1.90 times and showed an overall decreasing pattern as time increased. Phosphorylated ERK also increased 2.0 times according to the change of PKC¥å. Integrin ¥á7 increased 2.32 times and showed an increasing pattern as time increased. In conclusion, naltrexone influences PKC¥å neuronal signaling system and endothelial adhesion molecule integrin ¥á7 in addition to the well-known opioid system.
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KEYWORD
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